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Part:BBa_K1463032:Design

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-09-09)


Suffix and extra nucleotides for recombinase switch function


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Extra nucleotides contain restriction site to check for switch function.


Source

Synthesised by IDT from a given sequence.

References